High-dimensional single-cell landscape of immune alterations during HTLV-1 infection and leukemogenesis identifies hallmarks of premalignant and malignant T-cell states and the accompanying shift of systemic immune state toward myeloid and immunosuppressive.

Clonal ZBTB33 mutations are detected within blood sequencing datasets of healthy donors and in several MDS patients, identifying ZBTB33 as a candidate driver of clonal hematopoiesis. Mouse hematopoietic stem cells with Zbtb33 mutations exhibit clonal expansion and changes in RNA splicing.

A subset of pediatric AML cases harbors more somatic mutations in their genomes compared to normal blood progenitors. This subset displays expression profiles that resemble more committed progenitors and associates with better patient survival.